Study of serological techniques for diagnosis of Brucella abortus infection in buffaloes (Bubalus bubalis) / Estudo comparativo de diferentes técnicas sorológicas para diagnóstico de infecções por Brucella abortus em búfalos (Bubalus bubalis)

AUTOR(ES)

Lilia Marcia Paulin Silva

DATA DE PUBLICAÇÃO

2006

RESUMO

Considering the importance of the buffalo as a protein source in Brazil and the National Program of Brucelosis and Tuberculosis Control and Eradication for the evolution of the bovine and bubaline sector, and also due to the few number of studies on serological diagnosis of brucelosis in this specie, procedure that supports the process of certification of brucelosis free herds, the present study aimed to compare different serological tests for brucelosis diagnosis on buffaloes. Serum samples from 696 adult bubaline females had been analyzed to BPA, AATE, AAT (qualitatives), SRP, SLT, ELISAI, ELISAC, PF and ME and FC (quantitatives). The combination of two tests, FC and ME was used as reference, considering two criteria: a) positive animals: reactors for the FC (titer ≥ 20UI) and ME (vaccinated females), negative animals: no reactors for the FC (titer <20UI) and ME (vaccinated females); b) positive animals: reactors for the FC (titer ≥ 20UI) and ME (not vaccinated females), negative animals: no reactors for the FC (titer <20UI) and ME (not vaccinated females). It was necessary because was not possible to garantee the true vaccination status of the animals. The first step was performing the ROC analysis for all the quantitative tests (SRP, SLT, ELISAC, ELISAI and PF), and so the relative sensitivity and specificity and the Kappa were calculated for each test. Conclusions: a) the cut offs of ELISAC and PF tests proposed for bovines can be used for bubalines with reasonable security; b) specific cut offs for buffaloes to be used in SLT, SRP and ELISAI must be established; c) the best relative sensitivity and specificity had been reached by the ELISAC, PF, BPA and AAT; d) the best results of the Kappa had been reached by ELISAC (kappa), PF, BPA, AATE and AAT; e) BPA, AAT and AATE are the best screening diagnosis test for bubalines and ELISAC and PF are the most promising confirmatory tests for this species.

ASSUNTO(S)

buffaloes brucelose sorological diagnosis sorodiagnóstico brucellosis búfalos

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