Estudos dos efeitos citotoxicos e de estresse oxidativo induzido pelo cloreto de cadmio associado ou não ao sulfato de zinco em celulas musculares esqueleticas e neoplasicas / Study of citotoxic effects and oxidative stress induced by cadmium chloride associated or not to zinc sulfate in skeletal muscle and neoplasic cells

AUTOR(ES)

Claudia Lumy Yano

DATA DE PUBLICAÇÃO

2006

RESUMO

The heavy metals as cadmium are a toxic agent since it is extensively utilized in industry and can be amply distributed in environment. The cadmium is research focused as its pathological consequences in human exposure as it has been classified as carcinogenic agent. This fact is evident since the cadmium inhalation can be related to lung tumour and many studies show the possible participation of the cadmium on tumoral cells initiation and progression. However, few studies observed that cadmium can be accumulated in animal skeletal muscle cells and its action mechanisms are not completed known. The cadmium chloride exposure promoted oxidative stress and morphologic changes in C2C12 myotubes cell, in vitro, associated to decrease on cellular viability, high lipid peroxidation (increase on malondialdehyde content, MDA) and decrease on glutathione-S-transferase (GST) activity. The cadmium chloride produced chances on the cellular adhesion, integrity and retraction in C2C12 myotubes cells. These effects could be attenuated by zinc sulphate pre-treatment, which maintained the cellular viability, GST activity, reducing the MDA content. The zinc sulphate pre-treatment preserved the cellular adhesion, especially in high cadmium chloride concentration. Additionally, the tumoral cells (colon adenocarcinoma MAC 13) chronically exposed to cadmium chloride showed increase on the mitochondrial activity, and reduction on lysosomal and cellular viability, especially at high cadmium chloride concentration after 24h of treatment, probably indicating the tumoral cell changes

ASSUNTO(S)

sulfato de zinco cadmium chloride zinc sulfate cloreto de cadmio celulas musculares esqueleticas skeletal muscle cells carcinogenos carcinogenis

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