Structure of psoralen-crosslinked ribosomal RNA from Drosophila melanogaster.

AUTOR(ES)

Wollenzien, P L

RESUMO

Ribosomal RNA from Drosophila melanogaster photoreacted with hydroxymethyltrioxsalen has been examined by electron microscopy. Reproducible patterns of hairpins were found in both the 26S and 18S RNA. The frequency of these hairpins and the amount of incorporated drug were dependent upon the conditions under which the crosslinking was performed. A prominent central hairpin occurs in the 26S RNA and the break that interrupts the continuity of the RNA chain is located within it. In addition to several small hairpins, the crosslinked 18S RNA contains a large open loop.

Documentos Relacionados

• Psoralen-crosslinked secondary structure map of single-stranded virus DNA.
• RecA-dependent incision of psoralen-crosslinked DNA by (A)BC excinuclease.
• The structure of tRNA 5 Lys from Drosophila melanogaster.
• Complete cDNA sequence encoding mitochondrial large ribosomal RNA of Drosophila melanogaster.
• Preferential DNase I sensitivity of insert-free ribosomal RNA repeats of Drosophila melanogaster.