Structure and comparison of the oxytocin and vasopressin genes from rat.
AUTOR(ES)
Ivell, R
RESUMO
The gene encoding the precursor protein to the hormone oxytocin and its associated neurophysin has been isolated from a rat genomic library, and its sequence has been determined. The small gene (approximately equal to 850 base pairs) predicts a mRNA of approximately equal to 500 bases [without the poly(A) tail]. The exon-intron organization is similar to that of the vasopressin gene, with two splice sites in the protein-coding region. The first exon (A) comprises the 5' noncoding promoter region, a putative signal peptide, the nonapeptide hormone oxytocin, and the NH2-terminal, variable region of neurophysin. The second exon (B) encodes the central, conserved region of neurophysin, and the third exon (C) encodes the remaining COOH terminus of neurophysin, with an additional arginine residue at its end, presumably cleaved off during post-translational processing. A stretch of 143 nucleotides within exon B, except for a single base change, is entirely homologous to the equivalent part of the rat vasopressin gene, offering support for a gene conversion event having recently affected the two genes.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=345425Documentos Relacionados
- A synergistic effect of oxytocin and vasopressin on sodium excretion in the neurohypophysectomized rat.
- Vasopressin, oxytocin, dynorphin, enkephalin and corticotrophin-releasing factor mRNA stimulation in the rat.
- Oxytocin receptors on oxytocin neurones: histoautoradiographic detection in the lactating rat.
- Conformation of Lysine Vasopressin: A Comparison with Oxytocin
- Cholecystokinin evokes secretion of oxytocin and vasopressin from rat neural lobe independent of external calcium.