Strong minor groove base conservation in sequence logos implies DNA distortion or base flipping during replication and transcription initiation
AUTOR(ES)
Schneider, Thomas D.
FONTE
Oxford University Press
RESUMO
The sequence logo for DNA binding sites of the bacteriophage P1 replication protein RepA shows unusually high sequence conservation (∼2 bits) at a minor groove that faces RepA. However, B-form DNA can support only 1 bit of sequence conservation via contacts into the minor groove. The high conservation in RepA sites therefore implies a distorted DNA helix with direct or indirect contacts to the protein. Here I show that a high minor groove conservation signature also appears in sequence logos of sites for other replication origin binding proteins (Rts1, DnaA, P4 α, EBNA1, ORC) and promoter binding proteins (σ70, σD factors). This finding implies that DNA binding proteins generally use non-B-form DNA distortion such as base flipping to initiate replication and transcription.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=96701Documentos Relacionados
- The P1 phage replication protein RepA contacts an otherwise inaccessible thymine N3 proton by DNA distortion or base flipping
- Sequence Dependencies of DNA Deformability and Hydration in the Minor Groove
- DNA sequence preferences of several AT-selective minor groove binding ligands.
- TBP-DNA interactions in the minor groove discriminate between A:T and T:A base pairs.
- Protein and drug interactions in the minor groove of DNA