STRAND-SPECIFIC TRANSFER OF DONOR DNA DURING CONJUGATION IN E. coli*
AUTOR(ES)
Ihler, Garret
RESUMO
After conjugation of E. coli, only the preformed donor DNA strand with a leading 5′ end can be recovered from the recipient. DNA that was transferred to recipients from radioactive-labeled donor strains lysogenic for λ bacteriophage was recovered as radioactive material in mature phage particles. The radioactivity was recovered in strand l when gene transfer was in the order gal-λ-bio, and in strand r when the order of transfer was bio-λ-gal. The assignment of the 5′ end as the origin of the transferred strand follows directly from the known orientation of prophage λ in the chromosome.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=534013Documentos Relacionados
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