Stochastic model of protein–protein interaction: Why signaling proteins need to be colocalized
AUTOR(ES)
Batada, Nizar N.
FONTE
National Academy of Sciences
RESUMO
Colocalization of proteins that are part of the same signal transduction pathway via compartmentalization, scaffold, or anchor proteins is an essential aspect of the signal transduction system in eukaryotic cells. If interaction must occur via free diffusion, then the spatial separation between the sources of the two interacting proteins and their degradation rates become primary determinants of the time required for interaction. To understand the role of such colocalization, we create a mathematical model of the diffusion based protein–protein interaction process. We assume that mRNAs, which serve as the sources of these proteins, are located at different positions in the cytoplasm. For large cells such as Drosophila oocytes we show that if the source mRNAs were at random locations in the cell rather than colocalized, the average rate of interactions would be extremely small, which suggests that localization is needed to facilitate protein interactions and not just to prevent cross-talk between different signaling modules.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=404064Documentos Relacionados
- Protein-protein interaction: a genetic selection for compensating mutations at the barnase-barstar interface.
- Protein–protein interaction in insulin signaling and the molecular mechanisms of insulin resistance
- Phytochrome Phosphorylation Modulates Light Signaling by Influencing the Protein–Protein InteractionW⃞
- Use of Immunomatrix Methods to Improve Protein-Protein Interaction Detection
- Modular decomposition of protein-protein interaction networks