Stimulation of tubulin gene transcription by deciliation of sea urchin embryos.

AUTOR(ES)

Gong, Z Y

RESUMO

Deciliation by hypertonic shock of embryos of the sea urchin Lytechinus pictus resulted in an increase in synthesis of alpha- and beta-tubulins, the consequence of an increased concentration of RNA encoding the tubulins. RNA run-on assays in isolated nuclei indicated that this response is due to a transient increase in the rate of synthesis of tubulin RNA beginning within 5 min of deciliation. This enhancement of tubulin gene transcription also occurred in deciliated embryos treated with the microtubule-depolymerizing agent colcemid; thus the reaction to deciliation is not a response to a reduction in concentration of unpolymerized tubulin utilized for ciliogenesis. In deciliated embryos treated with colcemid, the elevated level of tubulin RNA declined rapidly, due to its destabilization by the elevated concentration of unpolymerized tubulin. The increased transcription of tubulin genes is a response to the loss of cilia, not to the hypertonic shock, and occurs even when cilium regeneration is prevented. Inhibition of protein synthesis with puromycin or emetine did not prevent the transcriptional enhancement but stabilized tubulin mRNA, resulting in increased accumulation of tubulin mRNA after deciliation.

Documentos Relacionados

• Stabilization of tubulin mRNA by inhibition of protein synthesis in sea urchin embryos.
• Actin gene expression in developing sea urchin embryos.
• Modulation of nucleosome structure by histone subtypes in sea urchin embryos.
• Translation of nonpolyadenylylated messenger RNA of sea urchin embryos.
• Four sizes of transcript produced by a single sea urchin gene expressed in early embryos.