Stabilization of a Full-Length Infectious cDNA Clone of Transmissible Gastroenteritis Coronavirus by Insertion of an Intron
AUTOR(ES)
González, José M.
FONTE
American Society for Microbiology
RESUMO
The stable propagation of a full-length transmissible gastroenteritis coronavirus (TGEV) cDNA in Escherichia coli cells as a bacterial artificial chromosome has been considerably improved by the insertion of an intron to disrupt a toxic region identified in the viral genome. The viral RNA was expressed in the cell nucleus under the control of the cytomegalovirus promoter and the intron was efficiently removed during translocation of this RNA to the cytoplasm. The insertion in two different positions allowed stable plasmid amplification for at least 200 generations. Infectious TGEV was efficiently recovered from cells transfected with the modified cDNAs.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=155106Documentos Relacionados
- Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus
- A Drosophila full-length cDNA resource
- Human delta-aminolevulinate dehydratase: nucleotide sequence of a full-length cDNA clone.
- Isolation and characterization of a rice full-length cDNA clone encoding a polyubiquitin.
- Isolation of a full-length cDNA clone encoding human tyrosine hydroxylase type 3.