Stability of Insertion Sequence IS1245, a Marker for Differentiation of Mycobacterium avium Strains
AUTOR(ES)
Bauer, Jeanett
FONTE
American Society for Microbiology
RESUMO
Recently a novel insertion element, IS1245, has been described and suggested for use as a probe in restriction fragment length polymorphism studies of Mycobacterium avium strains. An important issue in this context is the stability of the insertion element. We analyzed single colonies of M. avium cultures and found frequent small one- to two-band changes. However, following repeated in vitro passages over 1 year, similar one- to two-band changes were observed in the IS1245 patterns of only six M. avium strains investigated.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=84334Documentos Relacionados
- A novel insertion element from Mycobacterium avium, IS1245, is a specific target for analysis of strain relatedness.
- Estudo do mecanismo de transferência horizontal da sequência de inserção IS1245, específica de Mycobacterium avium, para Mycobacterium kansasii
- Characterization of IS1245 for Strain Typing of Mycobacterium avium
- Sequence-based differentiation of strains in the Mycobacterium avium complex.
- IS1245 Restriction Fragment Length Polymorphism Typing of Mycobacterium avium Isolates: Proposal for Standardization