Srd1, a Saccharomyces Cerevisiae Suppressor of the Temperature-Sensitive Pre-Rrna Processing Defect of Rrp1-1
AUTOR(ES)
Fabian, G. R.
RESUMO
We define a new gene, SRD1, involved in the processing of pre-rRNA to mature rRNA. The SRD1 gene was identified by selecting for second-site suppressors of the previously described rrp1-1 mutation. The rrp1-1 mutation causes temperature-sensitive growth, a conditional defect in processing of 27S pre-rRNA to mature 25S rRNA, and a nonconditional increase in sensitivity to several aminoglycoside antibiotics. All srd1 alleles identified are recessive and apparently specific to the rrp1-1 mutation. Although a mutation of SRD1 suppresses the pre-rRNA processing defect, drug sensitivity and thermolethality of a point mutation of RRP1, it is unable to suppress a rrp1-disruption allele. We suggest that the SRD1 gene product either interacts with or regulates the RRP1 product.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1203943Documentos Relacionados
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