Spore germination, early development and some notes on the effects of in vitro culture medium on Frullania ericoides (Nees) Mont. (Frullaniaceae, Marchantiophyta)
AUTOR(ES)
Silva-e-Costa, Juliana da Costa, Luizi-Ponzo, Andrea Pereira, Resende, Cristiano Ferrara de, Peixoto, Paulo Henrique Pereira
FONTE
Acta Bot. Bras.
DATA DE PUBLICAÇÃO
30/01/2017
RESUMO
ABSTRACT In bryophytes, establishment can occur by a sexual or asexual process, but the production of spores enables colonization of a wider range of habitats and substrates than can asexual propagules. Successful germination is critical for establishment in a new environment. This paper addresses germination and sporeling development in Frullania ericoides, a leafy liverwort species. Fresh spores were inoculated in vitro in different culture strengths of Knop’s nutrient solution (one-fourth strength, half strength, full strength, one and a half strength and double strength), in order to evaluate the effects of this solution on spore germination and on the development of external protonema. On the first assessment, spore germination was observed at all the concentrations. Germination was endosporic, with cell division and proliferation, resulting in a globular protonema, within the spore wall. Beginning at the fourth week, the development of tightly concave primordial leaves was observed in all but the double-strength medium. Throughout the period of study, the treatments with lower concentrations exhibited external protonema with greater lengths. The double-strength treatment was statistically different from other treatments in at least two parameters. The results of this study demonstrate the potential of in vitro culture techniques for bryophyte spore studies and germplasm preservation.
Documentos Relacionados
- Elimination of the adverse effects of urea fertilizer on seed germination, seedling growth, and early plant growth in soil
- Physiological Studies on Spore Germination, with Special Reference to Clostridium botulinum: III. Carbon Dioxide and Germination, with a Note on Carbon Dioxide and Aerobic Spores 1
- Factors influencing Clostridium botulinum spore germination, outgrowth, and toxin formation in acidified media.
- Factors Influencing Spore Germination and Early Gametophyte Development in Anemia mexicana and Anemia phyllitidis1
- Some Notes on the Early Adrenals1