Specificity and Evolutionary Divergence of the Antigenic Structure of the Polypeptide Chain Elongation Factors

AUTOR(ES)

Gordon, Julian

RESUMO

Antisera have been prepared against two electrophoretically homogeneous “polypeptide chain elongation factors,” T and G, from Escherichia coli. Inactivation and precipitation tests showed that these two fractions were antigenically distinct with no cross-reaction. The immune inactivation curve of G factor from E. coli was distinguishable from that of G factor from Pseudomonas fluorescens. Mammalian factors were not inhibited by antibody directed against the E. coli T and G factors. By Ouchterlony diffusion tests, the antisera also detected significant antigenic variability among different bacterial species. It is concluded that the factors have undergone considerable evolutionary divergence in their antigenic structure.

Documentos Relacionados

• Chromosomal Localization of the Structural Genes of the Polypeptide Chain Elongation Factors
• Polypeptide Chain Initiation and Stepwise Elongation with Artemia Ribosomes and Factors*
• Function–structure analysis of proteins using covarion-based evolutionary approaches: Elongation factors
• Evolutionary Divergence of an Elongation Factor 3 from Cryptococcus neoformans
• The Antigenic Structure and Specificity of Luminous Bacteria