Species-specific cellular DNA-binding proteins expressed in mouse cells transformed by chemical carcinogens.
AUTOR(ES)
Sen, A
RESUMO
Mouse cells transformed by DNA and RNA tumor viruses and by chemical carcinogens have been examined for the presence of specific DNA-binding proteins by DNA-cellulose chromatography. Using mouse DNA-cellulose we have obtained single-stranded DNA-binding proteins from two clones transformed by chemical carcinogens. Simian virus 40 transformants also have a DNA-binding protein [the tumor (T) antigen] that binds to mouse and human DNA with comparable affinity. Mouse sarcoma virus-transformed cells and two other chemically transformed clones showed no difference in DNA-binding protein pattern compared to the untransformed parental cell. The DNA-binding proteins isolated from the chemically transformed cell clones are between 25,000 and 30,000 daltons by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. These cellular "T proteins" bind to the homologous mouse cellular DNA with a higher affinity than to heterologous human cellular DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=392395Documentos Relacionados
- Species-specific phosphorylation of mouse and rat p53 in simian virus 40-transformed cells.
- Rapid isolation of specific DNA-binding proteins and their DNA-binding domains.
- Patterns of Viral DNA Integration in Cells Transformed by Wild Type or DNA-Binding Protein Mutants of Adenovirus Type 5 and Effect of Chemical Carcinogens on Integration
- UV-induced DNA-binding proteins in human cells.
- DNA-binding proteins in the cytoplasm of vaccinia virus-infected mouse L-cells.
