Sorologic evaluation of swine cysticercosis by ELISA using antigens: total extract and scolex from Cysticercus cellulosae and vesicular fluid from Cysticercus longicollis / Avaliação sorológica da cisticercose suína pelo ELISA utilizando os antígenos: total e de escólex de Cysticercus cellulosae e líquido vesicular de Cysticercus longicollis

AUTOR(ES)
DATA DE PUBLICAÇÃO

2003

RESUMO

Swine cysticercosis is a disease caused by the Taenia solium’s metacestodes, called Cysticercys cellulosae (Cisticerci). This disease is largely spread around the world and is more frequent in countries where swine meat consumption is high and sanitary conditions are poor, as in developing countries. Cysticercosis is harmful to the raising of the swine because infected meat is not proper for human consumption. To diagnose swine cysticercosis one clinically examines the tonge in vivo and also examines post-mortem (necropsy). The tonge exam has little sensitivity in spite of its high specificity as the parasite location cannot be determined. Post-mortem analysis is based on metacestodes’s favorite sites. Therefore, the heart, the tonge, the diaphragm, and the chewing muscles (masseteres and pterigoidis) in the tissue are analyzed in the regular sanitary inspection. However, mild infections, with a minor number of metacestodes in the tissues may not be diagnosed in the necropsy, showing that evidently there is an low sensitivity. Recently there has been suggestions of immunological tests, including the ELISA (Enzyme Linked Immunossorbent Assay) which has as a principle the antibodies research in swine’s serum. Thus, this study’s aim is to evaluate the antigens in the ELISA method for immunodiagnosis of the swine cysticercosis. The experiment used 7 weaned swine for technological rising were orally infected with 200.000 T. solium eggs. At every 7 days, up to the 140º day of infection, 10 mL of blood was collected and the serum was frozen at minus 20ºC. The serum were analyzed through the ELISA and the analyses used 3 antigens: total (T-Tso) and scolex (Es-Tso) of C. cellulosae and C. longicollis’s vesicular fluid (LV-Tcra). The in vivo tongue exam showed low sensitivity because at no time during in the infection the presence of metacestodes could be detected. Through the post-mortem exam one could notice the cysticercosis in all animals. However, the amount of metacestodes per animal was very low, considering the high number of inoculated eggs. As a total 238 metacestodes were detected and all were viable. As for distribution, one observed that the muscles of front and hindquarters showed more parasites with 24,38 % (58) and 28,57% (68), respectively. Only 18,67% (43) of metacestodes were found in tissue appointed for meat inspection. As for the ELISA, the standardization was obtained for 3 antigens, that detected a significant rise of antibodies from the 21th day of infection. It was also observed that the test using the 3 antigens could detected antibodies anti-Cysticercus from the 21th day post-infection. The highest rate of reactivity was found for the antigen LV-Tcra. The immunoenzimatic tests with the Es-Tso and LV-Tcra showed best results, detecting antibodies level over the cut-off in the animals. In the analysis of the suspected animals with ELISA using Es-Tso, 64,3% (9) samples showed high absorbance to the cut-off and the animals considered probably cysticercosis carriers. In conclusion, it was proved that there is good sensitivity in the ELISA, particularly when the antigen Es-Tso is used, detecting cysticercosis in animals with mild infection. Moreover, the ELISA with the Es-Tso show as an important tool for the cysticercosis epidemiological research.

ASSUNTO(S)

cisticercose suína taenia crassiceps antígenos elisa swine cysticercosis taenia solium elisa antigens taenia crassiceps taenia solium

ACESSO AO ARTIGO

Documentos Relacionados