Sodium alendronate, atorvastatin calcic and flavonoids in osteoporosis induced by glucocorticoids / Alendronato de sódio, atorvastatina cálcica e flavonóides na osteoporose induzida por glicocorticóide

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

The objective of this paper is to evaluate the influence of sodium alendronate, atorvastatin calcic, ipriflavone and rutin in isolation and in combination, in osteoporosis induced for the administration of dexamethasone (dose of 7mg/kg of weight), in female Wistar rats (Rattus norvegicus albinus). The dosages of sodium alendronate, atorvastatin calcic, ipriflavone and rutin, were of 0,02 mg, 0,3 mg, 50 mg e 25 mg, respectively; and all the substances were administered orally, daily. After the induction period of osteoporosis, the treatments began. In the first experiment, the animals were distributed in five groups: G1: control, G2: control with osteoporosis (animal had received only dexamethasone), G3: dexamethasone + alendronate, G4: dexamethasone + atorvastatin and G5: dexamethasone + ipriflavone. In the second experiment, the animals were distributed in six groups: G1: control, G2: control with osteoporosis, G3: dexamethasone + alendronate, G4: dexamethasone + alendronate + atorvastatin, G5: dexamethasone + alendronate + ipriflavone and G6: dexamethasone + alendronate + rutin. In the third experiment, the animals were distributed in six groups: G1: control, G2: control with osteoporosis, G3: dexamethasone + alendronate + ipriflavone + rutin, G4: dexamethasone + ipriflavone + rutin, G5: dexamethasone + rutin and G6: dexamethasone + atorvastatin + ipriflavone. By the 10th, 20th and 30th days, after the beginning of the treatments, samples were collected of blood for dosages of calcium, phosphorus, magnesium and glucose; of left tibia for collagenous protein and non collagenous protein analysis and of left femur for histomorphometric analysis. In the first experiment, the results did not show significant differences between the control group and the control group with osteoporosis, or even between them and the treated groups in relation to the levels of calcium and magnesium; collagenous bone protein and cortical thickness. At the end of the experimental period a significant reduction was observed in the serum levels of phosphorus, of non collagenous bone protein and in the number of bone trabeculae in the control group with osteoporosis when compared with the control group. It was also observed that all the groups treated presented significant increases in the levels of non collagenous protein and that only the group treated with ipriflavone presented a significant increase in the number of bone trabeculae, when compared with the control group with osteoporosis. In the second experiment the results did not show any significant differences between the control group and the control group with osteoporosis, or even between them and the groups treated relating to the serum levels of calcium, phosphorus and magnesium. For the levels of blood glucose it was observed that dexamethasone induced hyperglycemia and only the group which received alendronate together with ipriflavone reduced the glycemia. For the non collagenous proteins only the group which received alendronate together with atorvastatin presented any significant increase in relation to the control groups. The results showed a significant reduction in the levels of collagenous protein in the control group with osteoporosis, but no alteration was observed between it and the treated groups. A significant reduction was also observed in the number of bone trabeculae and in the cortical thickness in the control group with osteoporosis, considering that all the groups treated showed significant increases in the number of trabeculae at the end of the experimental period and only the group which received alendronate together with ipriflavone, did not present significant differences in cortical thickness. In the third experiment the results did not show significant differences between the control group and the control group with osteoporosis in relation to the serum levels of calcium, phosphorus and magnesium; bone levels of collagenous protein and non collagenous protein; considering that all of the treatments reduced the serum levels of calcium by the end of the experimental period. For the levels of blood glucose, it was observed that dexamethasone induced hyperglycemia and the groups treated with alendronate together with ipriflavone and rutin and rutin in isolation reduced the levels of glycemia when compared with the control group with osteoporosis. Through histomorphometric analysis it was observed that dexamethasone reduced the number of bone trabeculae and that all the substances tested were effective in increasing them, except the group which received ipriflavone together with rutin. Besides this, dexamethasone reduced cortical thickness and the groups which received alendronate together with ipriflavone and rutin and rutin in isolation presented significant increases in this parameter. In the face of these results it is concluded that rutin and ipriflavone seperately and ipriflavone used together with alendronate and rutin were effecticve in reducing the glycemia induced by the dexamethasone. Besides this, rutin, alendronate and ipriflavone seperately and together were effective in increasing the number of bone trabeculae and that alendronate, atorvastatin and ipriflavone, when administered seperately, and the association of alendronate with atorvastatin increase the levels of non collagenous proteins. In this way, these substances, whether used in isolation or in combination, could be promising in the treatment of osteoporosis induced by glucocorticoids.

ASSUNTO(S)

glicocorticóide glucocorticoids flavonóides osteoporose osteoporosis flavonoids bioquimica

ACESSO AO ARTIGO

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