Site-specific and photo-induced alkylation of DNA by a dimethylanthraquinone-oligodeoxynucleotide conjugate.
AUTOR(ES)
Kang, H
RESUMO
A dialkyl-substituted anthraquinone derivative was synthesized and ligated to a sequence-directing oligodeoxynucleotide to examine its efficiency and specificity for cross-linking to complementary sequences of DNA. The anthraquinone appendage stabilized spontaneous hybridization of the target and probe sequences through non-covalent interactions, as indicated by thermal denaturation studies. Covalent modification of the target was induced by exposure to near UV light (lambda > 335 nm) to generate cross-linked duplexes in yields as great as 45%. Reaction was dependent on the first unpaired nucleotide extended beyond the duplex formed by association of the target and probe. A specificity of C > T > A = G was determined for modification at this position. The overall site and nucleotide selectivity seems to originate from the chemical requirements of cross-linking and does not likely reflect the dominant solution structure of the complex prior to irradiation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=146215Documentos Relacionados
- Oligodeoxynucleotide-directed photo-induced cross-linking of HIV proviral DNA via triple-helix formation.
- Detection by electron microscopy of photo-induced denaturation in lambda DNA.
- Photo-induced cross-linkage of gene-5 protein and bacteriophage fd DNA+
- Photo-induced dipole relaxation current in natural Amethyst
- Site-specific initiation of a DNA fragment.
