Sinorhizobium meliloti Cells Require Biotin and either Cobalt or Methionine for Growth
AUTOR(ES)
Watson, Robert J.
FONTE
American Society for Microbiology
RESUMO
Sinorhizobium meliloti is usually cultured in rich media containing yeast extract. It has been suggested that some components of yeast extract are also required for growth in minimal medium. We tested 27 strains of this bacterium and found that none were able to grow in minimal medium when methods to limit carryover of yeast extract were used during inoculation. By fractionation of yeast extract, two required growth factors were identified. Biotin was found to be absolutely required for growth, whereas previously the need for this vitamin was considered to be strain specific. All strains also required supplementation with cobalt or methionine, consistent with the requirement for a vitamin B12-dependent homocysteine methyltransferase for methionine biosynthesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=93089Documentos Relacionados
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