Simple method for production of internal control DNA for Mycobacterium tuberculosis polymerase chain reaction assays.
AUTOR(ES)
deWit, D
RESUMO
A simple method for the production of internal control DNA for two well-established Mycobacterium tuberculosis polymerase chain reaction assays is described. The internal controls were produced from Mycobacterium kansasii DNA with the same primers but at a lower annealing temperature than that used in the standard assays. In both assays, therefore, the internal control DNA has the same primer-binding sequences at the target DNA. One-microgram quantities of internal control DNA which was not contaminated with target DNA could easily be produced by this method. The inclusion of the internal control in the reaction mixture did not affect the efficiency of amplification of the target DNA. The method is simple and rapid and should be adaptable to most M. tuberculosis polymerase chain reaction assays.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=265724Documentos Relacionados
- Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction.
- Rapid, simple method for typing isolates of Mycobacterium tuberculosis by using the polymerase chain reaction.
- Detection of Mycobacterium tuberculosis DNA in clinical samples by using a simple lysis method and polymerase chain reaction.
- Polymerase chain reaction for detection of Mycobacterium tuberculosis.
- DNA fingerprinting of Mycobacterium tuberculosis isolates by ligation-mediated polymerase chain reaction.
