Simple and Reliable Method To Precipitate Proteins from Bacterial Culture Supernatant
AUTOR(ES)
Caldwell, Randolph B.
FONTE
American Society for Microbiology
RESUMO
A simple and reliable method for precipitating protein from bacterial culture supernatants based on a pyrogallol red-molybdate-methanol (PRMM) protocol has been developed and applied for the analysis of proteins secreted by a bacterial type III secretion system. PRMM-based precipitation has been shown to be more efficient and robust than are conventional protocols.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321231Documentos Relacionados
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