SHORT-TERM N215-INCORPORATION BY AZOTOBACTER1
AUTOR(ES)
Bulen, W. A.
RESUMO
Bulen, W. A. (Charles F. Kettering Research Laboratory, Yellow Springs, Ohio), J. R. LeComte, and H. E. Bales. Short-term N215-incorporation by Azotobacter. J. Bacteriol. 85:666–670. 1963.—Short-term N215-incorporation measurements were used to determine which of the growth requirements were necessary for nitrogen fixation by Azotobacter agilis (A. vinelandii). Normal cells required neither added iron nor molybdenum, but a marked stimulation by Na+ and a minor stimulation by Mg2+ were observed. The Na+ stimulation was not accompanied by an increase in O2 uptake. A lag period preceded the response of molybdenum-deficient cells to added Mo. In systems employing 10 and 20% O2 with 10% N215 in the gas phase, O2 appeared to be both required and inhibitory. These observations may be helpful in attempts to fractionate cell-free nitrogen-fixing systems from this aerobe.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=278199Documentos Relacionados
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