Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCR
AUTOR(ES)
Zerva, L.
FONTE
American Society for Microbiology
RESUMO
Human brucellosis poses a significant public health problem in many developing countries and requires fast and accurate diagnosis. A PCR assay amplifying part of the 31-kDa Brucella abortus antigenic protein gene sequence was developed and applied to whole-blood and serum samples from 31 brucellosis patients and 45 healthy individuals. All patients except one had detectable Brucella DNA in either whole blood or serum (combined sensitivity, 97%), but the assay sensitivity was higher with serum samples (94%) than with whole-blood samples (61%). The assay specificity was excellent (100%). A confirmatory PCR assay targeting another Brucella gene region (omp-2) was also developed but lacked sensitivity. Serum is the optimal specimen for the diagnosis of brucellosis by PCR, a choice that leads to assay simplification and shortens turnaround time.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=87995Documentos Relacionados
- PCR Assay for Diagnosis of Human Brucellosis
- Rapid diagnosis of human brucellosis by peripheral-blood PCR assay.
- Diagnosis of recent and relapsed cases of human brucellosis by PCR assay
- Development and Evaluation of a PCR-Enzyme-Linked Immunosorbent Assay for Diagnosis of Human Brucellosis
- Convenience of Serum for Visceral Leishmaniasis Diagnosis by PCR
