Serotyping Isolates of Anaplasma phagocytophilum by Using Monoclonal Antibodies

AUTOR(ES)

Inokuma, Hisashi

FONTE

American Society for Microbiology

RESUMO

Ten mouse monoclonal antibodies (MAbs) that react with Anaplasma phagocytophilum (the human granulocytic ehrlichiosis agent) Webster isolates were developed. Seven different isolates of A. phagocytophilum were subtyped with these MAbs. Western blot analysis revealed that these MAbs reacted mainly with 41- to 46-kDa Msp2 proteins. Six MAbs reacted with all isolates. Four other MAbs reacted with human isolates from Wisconsin, but not with human isolates from New York or with animal isolates. Three different serotypes were identified. These features may lead to the development of other specific MAbs in order to provide tools for antigenic characterization of human isolates of A. phagocytophilum.

Documentos Relacionados

• Identification and serotyping of Microsporum canis isolates by monoclonal antibodies.
• Serotyping of Chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test.
• Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies.
• Development of Monoclonal Antibodies against Ureaplasma urealyticum Serotypes and Their Use for Serotyping Clinical Isolates
• Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses.