Seroreactivity to new Mycobacterium leprae protein antigens in different leprosy-endemic regions in Brazil
AUTOR(ES)
Hungria, Emerith Mayra, Oliveira, Regiane Morillas de, Souza, Ana Lúcia Osório Maroclo de, Costa, Maurício Barcelos, Souza, Vânia Nieto Brito de, Silva, Eliane Aparecida, Moreno, Fátima Regina Vilani, Nogueira, Maria Esther Salles, Costa, Maria Renata Sales Nogueira, Silva, Sônia Maria Usó Ruiz, Bührer-Sékula, Samira, Reed, Steven G, Duthie, Malcolm S, Stefani, Mariane Martins de Araújo
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2012-12
RESUMO
New Mycobacterium leprae protein antigens can contribute to improved serologic tests for leprosy diagnosis/classification and multidrug therapy (MDT) monitoring. This study describes seroreactivity to M. leprae proteins among participants from three highly endemic leprosy areas in Brazil: central-western Goiânia/Goiás (GO) (n = 225), Rondonópolis/Mato Grosso (MT) (n = 764) and northern Prata Village/Pará (PA) (n = 93). ELISA was performed to detect IgG to proteins (92f, 46f, leprosy IDRI diagnostic-1, ML0405, ML1213) and IgM to phenolic glycolipid-I (PGL-I). Multibacillary (MB) leprosy had positive rates for PGL-I that were similar to those for proteins; however, some anti-PGL-I-negative subjects were positive for proteins, suggesting that adding protein antigen to PGL-I can enhance the sensitivity of MB leprosy detection. In MT, different degrees of seroreactivity were observed and ranked for MB, former patients after MDT, paucibacillary (PB) leprosy, household contact (HHC) and endemic control (EC) groups. The seroreactivity of PB patients was low in GO and MT. HHCs from different endemic sites had similar IgG antibody responses to proteins. 46f and 92f were not recognised by most tuberculosis patients, ECs or HHCs within GO, an area with high BCG vaccination coverage. Low positivity in EC and HHC was observed in PA and MT. Our results provide evidence for the development of an improved serologic test that could be widely applicable for MB leprosy testing in Brazil.
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