Serological differentiation between infected and vaccinated cattle by using purified soluble antigens from Brucella abortus in a hemagglutination system.

AUTOR(ES)

Raybould, T J

RESUMO

Serological tests used in current brucellosis eradication schemes, such as bacterial tube agglutination, do not readily distinguish between infected animals and those immunized with strain 19 or 45/20 Brucella abortus vaccines. In this study, sera from naturally infected cattle were used to identify serologically important antigens in extracts of virulent B. abortus by gel diffusion techniques. Antisera from rabbits hyperimmunized with selected precipitation lines were used for purification by affinity chromatography of two precipitating and one non-precipitating antigen from crude bacterial extracts. A passive hemagglutination test using these antigens was developed. A number of characterized bovine sera were screened by passive hemagglutination and conventional bacterial tube agglutination test. A considerable improvement in discrimination between sera from infected and vaccinated cattle was obtained with the hemagglutination test compared with bacterial tube agglutination.

Documentos Relacionados

• Differentiation between Mycobacterium bovis BCG-Vaccinated and M. bovis-Infected Cattle by Using Recombinant Mycobacterial Antigens
• Enzyme-linked immunosorbent assay with partially purified cytosoluble 28-kilodalton protein for serological differentiation between Brucella melitensis-infected and B. melitensis Rev.1-vaccinated sheep.
• Brucella abortus 1119-3 O-chain polysaccharide to differentiate sera from B. abortus S-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion.
• Antibody responses to Brucella abortus 2308 in cattle vaccinated with B. abortus RB51.
• Comparison of an agar-gel immunodiffusion test with other serological methods for differentiating Brucella infected from vaccinated cattle.