Serodiagnosis of ocular toxocariasis: a comparison of two antigens.
AUTOR(ES)
Glickman, L T
RESUMO
This study was designed to compare the sensitivity and specificity of enzyme linked immunosorbent assay (ELISA) for the serodiagnosis of ocular toxocariasis using Toxocara canis embryonated egg antigen (TEE) and toxocara excretory-secretory or exoantigen (TEX) produced in vitro. TEE and TEX ELISA were comparably sensitive, but TEX ELISA was better able to discriminate between serum samples from patients with ocular toxocariasis and those from patients with retinoblastoma. In addition, preabsorption of sera with Ascaris suum embryonated egg antigen seemed to be essential to prevent false positive results with TEE ELISA but was not so critical for TEX-ELISA. Further studies are still required to standardise TEX for serodiagnosis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=499081Documentos Relacionados
- Experimental ocular toxocariasis: a mouse model.
- Human toxocariasis: a seroepidemiological survey in schoolchildren of Sorocaba, Brazil
- Serodiagnosis of Chagas' disease by enzyme-linked immunosorbent assay using two synthetic peptides as antigens.
- Toxocariasis: Clinical Aspects, Epidemiology, Medical Ecology, and Molecular Aspects
- Serodiagnosis of Streptococcus faecalis endocarditis by immunoblotting of surface protein antigens.