Serine Protease Inhibitors Block Invasion of Host Cells by Toxoplasma gondii
AUTOR(ES)
Conseil, V.
FONTE
American Society for Microbiology
RESUMO
We investigated the effect of protease inhibitors on the asexual development of the protozoan parasite Toxoplasma gondii. Among the inhibitors tested only two irreversible serine protease inhibitors, 3,4-dichloroisocoumarin and 4-(2-aminoethyl)-benzenesulfonyl fluoride, clearly prevented invasion of the host cells by specifically affecting parasite targets in a dose-dependent manner, with 50% inhibitory concentrations between 1 and 5 and 50 and 100 μM, respectively. Neither compound significantly affected parasite morphology, basic metabolism, or gliding motility within the range of the experimental conditions in which inhibition of invasion was demonstrated. No partial invasion was observed, meaning that inhibition occurred at an early stage of the interaction. These results suggest that at least one serine protease of the parasite is involved in the invasive process of T. gondii.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=89278Documentos Relacionados
- Toxoplasma gondii Homologue of Plasmodium Apical Membrane Antigen 1 Is Involved in Invasion of Host Cells
- Purification of Toxoplasma gondii from host cells.
- Attachment of Toxoplasma gondii to host cells is host cell cycle dependent.
- Host Cell Penetration of Toxoplasma gondii
- Interactions between Toxoplasma gondii and its host cells: function of the penetration-enhancing factor of toxoplasma.
