Sequences of 12 monoclonal anti-dinitrophenyl spin-label antibodies for NMR studies.

AUTOR(ES)

Leahy, D J

RESUMO

Eleven monoclonal antibodies specific for a spin-labeled dinitrophenyl hapten (DNP-SL) have been produced for use in NMR studies. They have been named AN01 and AN03-AN12. The stability constants for the association of these antibodies with DNP-SL and related haptens were measured by fluorescence quenching and ranged from 5.0 X 10(4) M-1 to greater than 1.0 X 10(8) M-1. cDNA clones coding for the heavy and light chains of each antibody and of an additional anti-DNP-SL monoclonal antibody, AN02, have been isolated. The nucleic acid sequence of the 5' end of each clone has been determined, and the amino acid sequence of the variable regions of each antibody has been deduced from the cDNA sequence. The sequences are relatively heterogeneous, but both the heavy and the light chains of AN01 and AN03 are derived from the same variable-region gene families as those of the AN02 antibody. AN07 has a heavy chain that is related to that of AN02, and AN09 has a related light chain. AN05 and AN06 are unrelated to AN02 but share virtually identical heavy and light chains. Preliminary NMR difference spectra comparing related antibodies show that sequence-specific assignment of resonances is possible. Such spectra also provide a measure of structural relatedness.

Documentos Relacionados

• Spin-label Studies of Membranes in Rye Protoplasts during Extracellular Freezing 12
• Spin-label studies of the excitable membranes of nerve and muscle.
• Spin-label study of hemoglobin conformations in solution.
• Spin-label electron spin resonance studies on the interactions of lysine peptides with phospholipid membranes.
• Determination of spin-label orientation within the myosin head.