Sequence and Transcripts of the Bacteriophage T4 DNA Repair Gene uvsY

AUTOR(ES)

Gruidl, Michael E.

RESUMO

We have cloned, sequenced and analyzed transcription of the phage T4 uvsY gene. This gene is transcribed from a single gp MotA-dependent middle promoter to give a major transcript of approximately 930 nucleotides and a minor transcript of approximately 620 nucleotides. All in vivo and in vitro uvsY transcripts show anomalous migration in agarose gels. The uvsY transcript contains an open reading frame coding for an 137 amino acid [15.8 kilodaltons (kD)] UvsY protein and two unidentified open reading frames, ORF UvsY.-1 (9.0 kD) and ORF UvsY.-2 (6.0 kD). Our DNA sequence differs in only three places from that published by Takahashi et al. However, one of these changes alters the predicted carboxy terminus of the UvsY protein. Marker rescue experiments map gene 25 to the region upstream of uvsY. Gene 25 is likely, although not certain, to correspond to an ORF that is found upstream from uvsY and is translated in the same direction.

Documentos Relacionados

• Confirmation of the reading frame of bacteriophage T4 uvsY gene.
• Isolation and Characterization of Conditional Alleles of Bacteriophage T4 Genes uvsX and uvsY
• Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.
• Genetic analysis of dar, uvsW, and uvsY in bacteriophage T4: dar and uvsW are alleles.
• Studies on the Recombination Genes of Bacteriophage T4: Suppression of uvsX and uvsY Mutations by uvsW Mutations