Sequence and cloning of bacteriophage T4 gene 63 encoding RNA ligase and tail fibre attachment activities.

AUTOR(ES)

Rand, K N

RESUMO

The sequence of gene 63 of bacteriophage T4 was determined by a shotgun approach. Small DNA fragments, derived by sonication of a restriction fragment that encompasses the region of gene 63, were cloned in M13 vectors and sequenced by the 'dideoxy' method. The position of the gene was established by comparison with the sequence of a gene 63 amber mutant. Knowledge of the DNA sequence of gene 63 and surrounding regions has allowed the construction of a clone of gene 63 in which RNA ligase production is under the control of the lac promoter of bacteriophage M13mp8. Infected E. coli cells can be induced to produce a protein indistinguishable from commercially available RNA ligase.

Documentos Relacionados

• Suppressors of mutations in the bacteriophage T4 gene coding for both RNA ligase and tail fiber attachment activities.
• Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate.
• Cloning, nucleotide sequence, and overexpression of the bacteriophage T4 regA gene.
• Nucleotide sequence of the tail sheath gene of bacteriophage T4 and amino acid sequence of its product.
• Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1