Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition
AUTOR(ES)
Khan, Abdul Basit, Khattak, Mehmood Iqbal, Tarar, Omer Mukhtar, Habib, Farzana, Jamil, Khalid, Yasmin, Arfa, Parvez, Shoukat
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2013-08
RESUMO
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks in FT-IR.
Documentos Relacionados
- Isolamento de bactérias produtoras de polihidroxialcanoatos e caracterização molecular de sua PHA sintase.
- Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons
- PhaC and PhaR Are Required for Polyhydroxyalkanoic Acid Synthase Activity in Bacillus megaterium
- Polyhydroxyalkanoate (PHA) Accumulation in Sulfate-Reducing Bacteria and Identification of a Class III PHA Synthase (PhaEC) in Desulfococcus multivorans
- Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates.