Separation of Chlorophylls c1 and c2 from Pigment Extracts of Pavlova gyrans by Reversed-Phase High Performance Liquid Chromatography 1

AUTOR(ES)

Fawley, Marvin W.

RESUMO

Chlorophylls c1 and c2 have been separated from total pigment extracts of the alga Pavlova gyrans Butcher using a reversed-phase high-performance liquid chromatography system. Pigments were separated on a 5 micrometer C18 column (25 centimeters × 4.6 millimeters) using a gradient of methanol-acetonitrile-water. Other photosynthetic pigments were also well resolved by the system used. The separation system described may replace current thin layer chromatography methods for qualitative and quantitative determination of chlorophyll c species.

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