Sensitivity of RT-PCR method in samples shown to be positive for Zika virus by RT-qPCR in vector competence studies
AUTOR(ES)
Paiva, Marcelo Henrique Santos, Guedes, Duschinka Ribeiro Duarte, Leal, Walter Soares, Ayres, Constância Flávia Junqueira
FONTE
Genet. Mol. Biol.
DATA DE PUBLICAÇÃO
22/05/2017
RESUMO
Abstract Tissue samples from mosquitoes artificially infected with Zika virus and shown to be positive by RT-qPCR were reexamined by RT-PCR. Using these samples we compared the two methods employed in virus RNA detection for vector competence studies. Results demonstrated that, albeit useful, RT-PCR gave false negatives with low viral loads (< 106 RNA copies/ml).
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