Sensitive high-pressure liquid chromatographic assay for amphotericin B which incorporates an internal standard.
AUTOR(ES)
Granich, G G
RESUMO
We developed a rapid, sensitive, high-pressure liquid chromatographic (HPLC) procedure which incorporates a commercially available internal standard, 1-amino-4-nitronaphthalene, to measure amphotericin B in serum. Recovery was quantitative (greater than or equal to 90%), and the standard curve was linear from 0.04 to at least 10.0 micrograms/ml. The reproducibility of the assay was good, with intrarun coefficients of variation from 2.0 to 6.8% and interrun coefficients of variation from 4.9 to 10.0%. Comparison by linear regression analysis of the HPLC assay with an agar well diffusion bioassay gave a correlation coefficient of 0.942, with the HPLC assay exhibiting greater precision and sensitivity. No interference was encountered from over 20 drugs and three amphotericin B analogs. However, serum specimens that contained high concentrations of conjugated bilirubin (greater than 3 mg/dl) produced interfering peaks in both this assay and other previously reported HPLC assays for amphotericin B. We also describe a solid-phase extraction procedure which effectively removes this interference and uses an alternative internal standard (N-acetyl amphotericin B).
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=180446Documentos Relacionados
- High-Pressure Liquid Chromatographic Assay of Netilmicin in Plasma
- High-Pressure Liquid Chromatographic Assay of Chloramphenicol in Biological Fluids
- High-pressure liquid chromatographic assay of ceftizoxime with an anion-exchange extraction technique.
- High-pressure liquid chromatographic quantitation of azlocillin.
- High-pressure liquid chromatographic method for analysis of cephalosporins.