Semen cool storage and cryopreservation of piau-aÃu Leporinus macrocephalus / Resfriamento e congelamento de sÃmen de piau-aÃu Leporinus macrocephalus

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The aim of this research was to develop a cool storage and cryopreservation protocol for piau-aÃu (Leporinus macrocephalus) semen. Sperm motility rate was used to access sperm quality after treatments. On experiment I, several solutions commonly used as extenders or sperm motility activators of fish semen, and NaCl and NaHCO3 at 25 and 200 mM were tested. On experiment II, DMSO, ethylene glycol, glycerol and methanol were tested as cryoprotectants during cool storage. On experiment III, semen samples were frozen after dilution on Kurokura solution (NaCl 128,4 mM, KCl 2,7 mM, CaCl2 1,4 mM, NaHCO3 2,4 mM) or NaCl 200 mM combined with 5 or 10% ethylene glycol, and Kurokura combined with 5, 10 or 15% methanol. NaCl 25 mM induced the highest motility rate and was used as sperm activator afterwards. Kurokura solution, NaCl 200 mM and Ginsburg fish Ringer (NaCl 123,2 mM, KCl 3,75 mM, CaCl2 3,0 mM, NaHCO3 2,65 mM) maintained sperm viability during 48 hours of cooling storage. Semen diluted on Kurokura combined or not with methanol produced motility rates above 80% after activation. All frozenthawed semen samples showed very low motility rates. The highest motility rate (8%) was obtained with samples diluted in Kurokura solution and 5% methanol. Piau-aÃu semen diluted in Kurokura solution maintain high sperm motility after 48-hour cooling. Due to the very low sperm motility after thawing, it is necessary to further investigate the effect of other cryoprotectants and extenders on cryopreservation of piau-aÃu semen.

ASSUNTO(S)

semen zootecnia preservaÃÃo piscicultura fish breeding preservation sÃmen reproduÃÃo reproduction

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