Selective enumeration of Fusobacterium necrophorum from the bovine rumen.

AUTOR(ES)

Tan, Z L

RESUMO

A culture medium containing lactate as the sole energy source and antibiotics (bacitracin, gentamicin, and streptomycin) was used for selection and enumeration of Fusobacterium necrophorum from bovine ruminal contents. F. necrophorum growth was determined by indole production, and enumeration was performed by the most-probable-number technique. The number of F. necrophorum cells in cattle fed a 100% forage diet was 7 x 10(5)/g of ruminal contents. The number increased (P < 0.05) 10-fold after the diet was changed to 85% corn grain.

Documentos Relacionados

• Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.
• Characteristics of methanogens isolated from bovine rumen.
• Glucose-1-phosphate as a selective substrate for enumeration of Bacteroides species in the rumen.
• Isolation and characterization of large treponemes from the bovine rumen.
• New medium selective for Fusobacterium species and differential for Fusobacterium necrophorum.