Selection of Fimbriate Transductants of Salmonella typhimurium Dependent on Motility
AUTOR(ES)
Old, D. C.
RESUMO
The ability to form type 1 fimbriae (Fim+) was readily transduced to 159 out of 161 wild-type motile Fim− FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim+ donor strain. Fim+ clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been enriched by culture in aerobic static broth for 48 to 96 hr. A Fim+ transductant was isolated from only 1 out of 280 tests made with 10 nonmotile recipient FIRN strains that were nonflagellate (Fla−)- or possessed “paralyzed” flagella (Fla+ Mot−), though motile variants from these strains were fully competent in yielding Fim+ transductants. The property of motility was thought to facilitate the selective outgrowth of Fim+ transductant bacteria by enabling them to migrate aerotactically to the surface of the broth where their fimbriae permitted them to float and grow in a pellicle stimulated by the free supply of atmospheric oxygen.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=246984Documentos Relacionados
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