SELEÇÃO DE ESPÉCIES DO GÊNERO Penicillium PRODUTORAS DE LIPASE LIGADA AO MICÉLIO PARA APLICAÇÃO EM HIDRÓLISE DE ÓLEOS VEGETAIS
AUTOR(ES)
Marotti, Braz S., Cortez, Daniela V., Gonçalves, Daniel B., Castro, Heizir F. de
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2017-05
RESUMO
Ten species of Penicillium genus isolated from different habitats were evaluated as mycelium bound lipase producers to be used in the hydrolysis of vegetable oils. Using olive oil as an inducer three species (P. italicum AT4421, P. janthinellum CCT3162 and P. purpurogenum AT2008) were able to produce lipases having high mycelium bound activities (>150 U g-1) and were further characterized in relation to their biochemical and kinetic properties and specificity using vegetable oils having majority fatty acids composition in C12:0 (coconut); C16:0 (palm); C18:1 (canola) and C18:1 (soybean). All the three lipases could enrich the medium with fatty acids according to their respective selectivity and the reaction hydrolysis was found to enhance at least three folds under ultrasonic irradiations. For P. purpurogenum lipase the highest hydrolysis degree (66.8 ± 0.2%) was attained with coconut oil. Both P. italicum and P. janthinellum lipases showed high selectivity for canola oil, resulting in hydrolysis degrees of 79.9 ± 0.5% and 63.5 ± 0.6%, respectively. Analysis of the hydrolysates confirmed that the majority of the fatty acids released by P. italicum and P. janthinellum lipases was composed by oleic acid, and P. purpurogenum lipase the hydrolysate contained approximately 50% of lauric acid.
Documentos Relacionados
- Produção de concentrados de ácidos graxos por hidrólise de óleos vegetais mediada por lipase vegetal
- Co-produção de lipase e biossurfactante em estado sólido para utilização em biorremediação de óleos vegetais e hidrocarbonetos
- Aplicação de resinas sulfônicas como catalisadores em reações de transesterificação de óleos vegetais
- Seleção de espécies/procedências do gênero Eucalyptus potenciais para o semi-árido do Brasil.
- Transformação metatetica de oleos vegetais em insumos para industria surfactantes