Secretion of Listeriolysin by Brucella suis Inhibits Its Intramacrophagic Replication
AUTOR(ES)
Köhler, Stephan
FONTE
American Society for Microbiology
RESUMO
The introduction into Brucella suis 1330 of a plasmid allowing the heterologous expression of a hybrid cytolysin containing listeriolysin from Listeria monocytogenes, and its export via the Escherichia coli hemolysin secretion pathway, resulted in secretion of active listeriolysin monitored by erythrocyte lysis. In contrast to observations with the nonhemolytic control strain, the phagosomes of infected human monocytes containing the hemolytic B. suis were partially disrupted, and this strain failed to multiply in human macrophage-like cells. These results added strong evidence supporting the proposal that the phagosome of the macrophage was the predominant niche of brucellae in their mammalian hosts.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=98220Documentos Relacionados
- Induction of dnaK through Its Native Heat Shock Promoter Is Necessary for Intramacrophagic Replication of Brucella suis
- Impairment of Intramacrophagic Brucella suis Multiplication by Human Natural Killer Cells through a Contact-Dependent Mechanism
- The analysis of the intramacrophagic virulome of Brucella suis deciphers the environment encountered by the pathogen inside the macrophage host cell
- CHARACTERIZATION OF INTRACELLULAR, GLUCOSIDIC POLYSACCHARIDE PRODUCED BY BRUCELLA SUIS
- Cellular fatty acids of Brucella canis and Brucella suis.