Screening of Escherichia coli Temperature-sensitive Mutants by Pretreatment with Glucose Starvation
AUTOR(ES)
Boyd, D.
RESUMO
A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis mutants and their classification into two general catagories. Complete protein synthesis mutants are unable to make any polypeptide material, whereas partial protein synthesis mutants are able to produce inactive proteins. The phenotypes of several mutants are described.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=252129Documentos Relacionados
- Escherichia coli mutants with a temperature-sensitive alcohol dehydrogenase.
- Temperature-sensitive autolysis-defective mutants of Escherichia coli.
- Temperature-Sensitive Osmotic Remedial Mutants of Escherichia coli
- Selection of Temperature-sensitive Activating Enzyme Mutants in Escherichia coli
- Temperature-sensitive 6-aminopenicillanic acid-resistant mutants of Escherichia coli.