Saccharomyces cerevisiae Sin3p facilitates DNA double-strand break repair
AUTOR(ES)
Jazayeri, Ali
FONTE
National Academy of Sciences
RESUMO
There are two main pathways in eukaryotic cells for the repair of DNA double-strand breaks: homologous recombination and nonhomologous end joining. Because eukaryotic genomes are packaged in chromatin, these pathways are likely to require the modulation of chromatin structure. One way to achieve this is by the acetylation of lysine residues on the N-terminal tails of histones. Here we demonstrate that Sin3p and Rpd3p, components of one of the predominant histone deacetylase complexes of Saccharomyces cerevisiae, are required for efficient nonhomologous end joining. We also show that lysine 16 of histone H4 becomes deacetylated in the proximity of a chromosomal DNA double-strand break in a Sin3p-dependent manner. Taken together, these results define a role for the Sin3p/Rpd3p complex in the modulation of DNA repair.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=341805Documentos Relacionados
- Aberrant Double-Strand Break Repair in rad51 Mutants of Saccharomyces cerevisiae
- A Role for Rev3 in Mutagenesis during Double-Strand Break Repair in Saccharomyces Cerevisiae
- Modulation of Saccharomyces Cerevisiae DNA Double-Strand Break Repair by Srs2 and Rad51
- Two pathways for removal of nonhomologous DNA ends during double-strand break repair in Saccharomyces cerevisiae.
- DNA motif associated with meiotic double-strand break regions in Saccharomyces cerevisiae