Role of the Phagocyte in Host-Parasite Interactions XII. Hydrogen Peroxide-Myeloperoxidase Bactericidal System in the Phagocyte
AUTOR(ES)
McRipley, R. J.
RESUMO
An antimicrobial system in polymorphonuclear neutrophils (PMN) consisting of myeloperoxidase and hydrogen peroxide has been proposed. This system appears to be activated during phagocytosis as a result of the stimulated metabolic activities. A lysed-granules (LG) fraction was prepared from guinea pig exudative PMN. LG alone possessed bactericidal activity which was related to the pH of the reaction; the lower the pH, the more marked the activity. When low concentrations of both H2O2 and LG were combined under conditions where neither factor alone exhibited significant killing power, there was a striking increase in bactericidal activity. This enhanced activity was much greater than an additive effect. The LG-peroxide antibacterial system was most active over a pH range of 4.0 to 6.0. The activity of the LG-peroxide system was essentially abolished by peroxidase inhibitors, NaN3, KCN, and aminotriazole. The antibacterial activity of this system was nonspecific in nature, being equally effective against gram-negative and gram-positive organisms.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=276842Documentos Relacionados
- Role of the Phagocyte in Host-Parasite Interactions XI. Relationship Between Stimulated Oxidative Metabolism and Hydrogen Peroxide Formation, and Intracellular Killing
- Role of the Phagocyte in Host-Parasite Interactions XXIV. Aldehyde Generation by the Myeloperoxidase-H2O2-Chloride Antimicrobial System: a Possible In Vivo Mechanism of Action
- Role of the Phagocyte in Host-Parasite Interactions XXVII. Myeloperoxidase-H2O2-Cl−-Mediated Aldehyde Formation and Its Relationship to Antimicrobial Activity
- Heads or Tails: Host-Parasite Interactions in the Drosophila-Wolbachia System
- Role of the Phagocyte in Host-Parasite Interactions VIII. Effect of Whole-Body X Irradiation on Nicotinamides, Lysosomal Enzymes, and Bactericidal Activities of Leukocytes During Phagocytosis