Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells.
AUTOR(ES)
Weinberg, A
RESUMO
Porphyromonas gingivalis is a periodontal pathogen capable of invading primary cultures of normal human gingival epithelial cells (NHGEC). Involvement of P. gingivalis fimbriae in the invasion process was examined. Purified P. gingivalis 33277 fimbriae blocked invasion of this organism into NHGEC in a dose-dependent manner. DPG3, a P. gingivalis fimbria-deficient mutant, was impaired in its invasion capability approximately eightfold compared to its parent, strain 381. However, adherence of the mutant was only 50% reduced compared to the parent. Biotin labeling of NHGEC surface proteins revealed that two fimbriated strains, but not DPG3, bound a 48-kDa NHGEC protein. Adhesin-receptor interactions, such as fimbriae binding to a 48-kDa NHGEC surface receptor, may trigger activation of eukaryotic proteins involved in signal transduction and/or provoke the generation of surface P. gingivalis molecules required for internalization.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=174594Documentos Relacionados
- Porphyromonas gingivalis invasion of gingival epithelial cells.
- A role for fimbriae in Porphyromonas gingivalis invasion of oral epithelial cells.
- Porphyromonas gingivalis Fimbriae Bind to Cytokeratin of Epithelial Cells
- Role of the Amino-Terminal Region of Porphyromonas gingivalis Fimbriae in Adherence to Epithelial Cells
- Interactions of Porphyromonas gingivalis with epithelial cells.
