RNA primer used in synthesis of anticomplementary DNA by reverse transcriptase of avian myeloblastosis virus.
AUTOR(ES)
Myers, J C
RESUMO
When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original RNA template that usually had adenosine at their 3' ends. When we used phage T/ RNA ligase (EC 6.5.1.3) to label the 3' end of the RNA template fragments contained in the RNA . cDNA hybrid intermediate, adenosine was found to be the principal nucleoside carrying the label. We infer from these results that the ribonuclease H (hybrid nuclease) activity of the reverse transcriptase creates fragments of the original RNA template with adenosine as the principal 3' terminus and that these fragments serve as primers for the synthesis of anticomplementary DNA.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=348485Documentos Relacionados
- Reverse transcriptase from avian myeloblastosis virus.
- Mechanism of RNA primer removal by the RNase H activity of avian myeloblastosis virus reverse transcriptase.
- Mechanism of interaction of avian myeloblastosis virus reverse transcriptase with avian myeloblastosis virus RNA.
- RNA-Dependent DNA Polymerase (Reverse Transcriptase) from Avian Myeloblastosis Virus: A Zinc Metalloenzyme
- Primer recognition by avian myeloblastosis virus RNA-directed DNA polymerase.
