RNA-Primed DNA Synthesis In Vitro
AUTOR(ES)
Keller, Walter
RESUMO
In vitro DNA synthesis on single-stranded circular DNA can be initiated by RNA primers. RNA chains are covalently extended by DNA polymerase II from KB cells and DNA polymerase I from Micrococcus luteus, but not by an RNA-dependent DNA polymerase from avian myeloblastosis virus. The reaction product consists of DNA chains with a piece of RNA at their 5′-ends, hydrogen bonded to the template DNA. The primer RNA is linked to the product DNA via a 3′:5′-phosphodiester bond, and can be specifically removed by ribonuclease H. The possible role of ribonuclease H in RNA-primed DNA synthesis in vivo is discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=426748Documentos Relacionados
- Initiation of RNA-primed DNA synthesis in vitro by DNA polymerase alpha-primase.
- RNA-primed DNA synthesis: specific catalysis by HeLa cell DNA polymerase alpha.
- Function of DNA polymerase I in RNA-primed synthesis of bacteriophage M-13 duplex DNA.
- RNA-primed complementary-sense DNA synthesis of the geminivirus African cassava mosaic virus.
- Initiation of simian virus 40 DNA replication in vitro: identification of RNA-primed nascent DNA chains.
