RNA-mediated ligation of self-cleavage products of a Neurospora mitochondrial plasmid transcript.

AUTOR(ES)

Saville, B J

RESUMO

Neurospora VS RNA is a mitochondrial single-stranded RNA that combines certain features of catalytic RNAs and group I introns. We report here that monomeric VS RNA synthesized in vitro by self-cleavage of a multimeric transcript can perform an RNA-mediated self-ligation reaction producing circular RNAs indistinguishable from those isolated from mitochondria. We conclude that the active site for the ligation reaction is present in the RNA itself. Also, the mechanism for aligning the termini to be ligated may be different from mechanisms previously described. The lack of sequence similarity between VS RNA and previously characterized catalytic RNAs suggests that VS RNA is an independently evolved ribozyme capable of both cleavage and ligation.

Documentos Relacionados

• Exceptionally fast self-cleavage by a Neurospora Varkud satellite ribozyme
• RNA conformational requirements of self-cleavage of hepatitis delta virus RNA.
• A computational approach to the mechanism of self-cleavage of hammerhead RNA.
• A chloroplast protein binds a viroid RNA in vivo and facilitates its hammerhead-mediated self-cleavage
• Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroid.