RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA

AUTOR(ES)

Vende, Patrice

FONTE

American Society for Microbiology

RESUMO

Phosphoprotein NSP5 is a component of replication intermediates that catalyze the synthesis of the segmented double-stranded RNA (dsRNA) rotavirus genome. To study the role of the protein in viral replication, His-tagged NSP5 was expressed in bacteria and purified by affinity chromatography. In vitro phosphorylation assays showed that NSP5 alone contains minimal autokinase activity but undergoes hyperphosphorylation when combined with the NTPase and helix-destabilizing protein NSP2. Hence, NSP2 mediates the hyperphosphorylation of NSP5 in the absence of other viral or cellular proteins. RNA-binding assays demonstrated that NSP5 has unique nonspecific RNA-binding activity, recognizing single-stranded RNA and dsRNA with similar affinities. The possible functions of the RNA-binding activities of NSP5 are to cooperate with NSP2 in the destabilization of RNA secondary structures and in the packaging of RNA and/or to prevent the interferon-induced dsRNA-dependent activation of the protein kinase PKR.

Documentos Relacionados

• A conserved double-stranded RNA-binding domain.
• Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase.
• RNA recognition by a Staufen double-stranded RNA-binding domain
• A new double-stranded RNA-binding protein that interacts with PKR
• The double-stranded RNA-binding protein X1rbpa promotes RNA strand annealing.