Reversible Oxidative Modification as a Mechanism for Regulating Retroviral Protease Dimerization and Activation

AUTOR(ES)

Davis, David A.

FONTE

American Society for Microbiology

RESUMO

Human immunodeficiency virus protease activity can be regulated by reversible oxidation of a sulfur-containing amino acid at the dimer interface. We show here that oxidation of this amino acid in human immunodeficiency virus type 1 protease prevents dimer formation. Moreover, we show that human T-cell leukemia virus type 1 protease can be similarly regulated through reversible glutathionylation of its two conserved cysteine residues. Based on the known three-dimensional structures and multiple sequence alignments of retroviral proteases, it is predicted that the majority of retroviral proteases have sulfur-containing amino acids at the dimer interface. The regulation of protease activity by the modification of a sulfur-containing amino acid at the dimer interface may be a conserved mechanism among the majority of retroviruses.

Documentos Relacionados

• A sequential dimerization mechanism for erythropoietin receptor activation.
• A reversible oxidative in activation of specific transfer RNA species.
• Kinetic stability as a mechanism for protease longevity
• Synthetic peptides as substrates and inhibitors of a retroviral protease.
• A ligand-reversible dimerization system for controlling protein–protein interactions