Reversed phase HPLC determination of tamoxifen in dog plasma and its pharmaco-kinetics after a single oral dose administration

AUTOR(ES)

Santana, Davi Pereira de, Braga, Rossana Maria Carvalho, Strattmman, Ruth, Albuquerque, Miracy Muniz, Bedor, Danilo César Galindo, Leal, Leila Bastos, Silva, José Alexsandro da

FONTE

Química Nova

DATA DE PUBLICAÇÃO

2008

RESUMO

The analytical method developed to evaluate tamoxifen in dog plasma samples was precise, accurate, robust and linear in the range of 5-200 ng/mL. The limits of detection and quantification were 0.981 ng/mL and 2.97 ng/mL, respectively. Besides, the intra-day precision and accuracy variations were 8.78 and 10.16%, respectively. Tamoxifen concentrations were analyzed by combined reversed phase liquid chromatography and UV detection (lambda=280 nm). The study was conducted using an open randomized 2-period crossover balanced design with a 1-week washout period between the doses. This simple, rapid and selective method is suitable for pharmacokinetic, bioavailability and bioequivalence studies.

Documentos Relacionados

• Determination of amitriptyline and its main metabolites in human plasma samples using HPLC-DAD: application to the determination of metabolic ratios after single oral dose of amitriptyline
• Simultaneous determination and pharmacokinetics of four coumarins in rat plasma after oral administration of traditional chinese medicine "YIGONG" capsule by SPE-HPLC
• Pharmacokinetics of Telithromycin in Plasma and Soft Tissues after Single-Dose Administration to Healthy Volunteers
• Pharmacokinetics of Amoxicillin: Dose Dependence After Intravenous, Oral, and Intramuscular Administration
• Pharmacokinetics of artesunate after single oral administration to rats