Retrovirus purification: method that conserves envelope glycoprotein and maximizes infectivity.
AUTOR(ES)
McGrath, M
RESUMO
A Sepharose 4B chromatographic method for purification of retroviruses is described which was less time consuming, increased purified virus yields, conserved viral glycoprotein, and increased recovery of biological infectivity in comparison with conventional sucrose gradient ultracentrifugation techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=525988Documentos Relacionados
- Vesicular stomatitis virus glycoprotein mutations that affect membrane fusion activity and abolish virus infectivity.
- Immunoaffinity purification and neutralization of scrapie prion infectivity.
- Natural immunity in mice to the envelope glycoprotein of endogenous ecotropic type C viruses: neutralization of virus infectivity.
- Truncation of the cytoplasmic domain of the simian immunodeficiency virus envelope glycoprotein increases env incorporation into particles and fusogenicity and infectivity.
- Identification of a site on herpes simplex virus type 1 glycoprotein D that is essential for infectivity.